B-10253

Yeast extract 0.30 g Na2-succinate 1.00 g (NH4)-acetate 0.50 g Fe(III) citrate solution (0.1% in H2O) 5.00 ml KH2PO4 0.50 g MgSO4 x 7 H2O 0.40 g NaCl 0.40 g NH4Cl 0.40 g CaCl2 x 2 H2O 0.05 g Vitamin B12 solution (10 mg in 100 ml H2O) 0.40 ml Trace element solution SL-6 (see below) 1.00 ml L-Cysteiniumchloride 0.30 g Resazurin(0,1%) 0.50 ml Distilled water 1000.00 ml Adjust pH to 6.8. Boil the medium for a few minute. Bubble the medium with nitrogen gas and fill 10 ml in 15 ml tubes with a rubber septum under a stream of nitrogen gas. Autoclave at 121C for 15 min. Sterile syringes are used to inoculate and remove samples. Incubate in the light using a tungsten lamp. Trace element solution SL-6: ZnSO4 x 7 H2O 0.10 g MnCl2 x 4 H2O 0.03 g H3BO3 0.30 g CoCl2 x 6 H2O 0.20 g CuCl2 x 2 H2O 0.01 g NiCl2 x 6 H2O 0.02 g Na2MoO4 x 2 H2O 0.03 g Distilled water 1000.00 m Boil the medium under a stream of nitrogen gas for few minutes and distribute 45 ml medium into 50 ml screw-capped bottles (already flushed with nitrogen gas). Bubble each bottle with nitrogen gas and close immediately with a rubber septum and screw tight. Autoclave at 121°C for 15 min. Sterile syringes are used to inoculate and remove samples. Incubate in the light using a tungsten lamp. For brown and other oxygen sensitive Rhodospirillaceae add 300 mg of L-cysteine (0.03% end concentration) to the boiling medium and readjust the pH to 6.8 or to the prepared medium in bottles inject neutralized sulfide solution (0.005 to 0.01% end concentration). The medium has been modified according to reference 3365. (продолжение см. Среда 319)